Table 2.

Vectors, restriction enzymes, and RNA polymerases used to produce sense and anti-sense probes for A. rubens neuropeptide precursor transcripts.

Precursor	Vector	Sense probe		Anti-sense probe
		Restriction endonuclease	RNA polymerase	Restriction endonuclease	RNA polymerase
S1	pBluescript SKII (+)	BamHI	T7	KpnI	T3
S2	pCR®-Blunt II-TOPO®	HindIII	T7	EcoRV	SP6
Asterotocin	pBluescript SKII (+)	SacI	T7	KpnI	T3
NGFFYamide	pBluescript SKII (+)	SacI	T7	KpnI	T3
TRH	pBluescript SKII (+)	SacI	T7	XhoI	T3
GnRH	pBluescript SKII (+)	XhoI	T3	BamHI	T7
Calcitonin	pBluescript SKII (+)	XbaI	T7	XhoI	T3
CRH	pBluescript SKII (+)	XbaI	T7	XhoI	T3