Ovarian gene expression measured by real time qPCR assays was indicated in panels A–F. The results showed many of the FSH-responsive genes suppressed in Fshb−/− mice remained the same in Fshb−/−
HSFHBdgc mice. HFSHBWT transgene restored the expression levels of many genes similar to that observed in ovaries of control (Ctrl.) mice. Each assay was performed on four cDNA samples and each sample run in triplicate. * P < 0.01 versus Ctrl. or Fshb−/−
HSFHBdgc groups; ** versus Fshb−/− group. qPCR assays to detect the expression of the following genes were used- Fshr: follicle-stimulating hormone-receptor, Cyp19a1: aromatase, Inhbb: activin beta b, Kcnj8: potassium inwardly-rectifying channel subfamily J member 8, S100a6: S100calcium binding protein A6 (calcyclin), Lhr: luteinizing hormone receptor.