Fig. 7.

Immunolabeling of ovaries obtained from mice at 21d of age showed that phospho-CREB (A) and phospho-PKA substrate (B) levels did not differ between Fshb^−/− and Fshb^−/− HSFHB^dgc mice indicating no rescue in contrast to that observed in Fshb^−/− HSFHB^WT mice. However, there were no differences in mitotically active phospho-Histone H3⁺ cells (C) in ovarian sections obtained from all genotypes of mice. Fshb^−/− HSFHB^dgc female mice, similar to Fshb^−/− females were infertile as indicated by mating trials that resulted in production of no pups in a 6 month-period. In contrast, the breeding performance of Fshb^−/− HSFHB^WT mice was identical to that observed in control mice (D and E) indicating full-functional rescue by the HSFHB^WT transgene.