Comparison of Fshb−/−
HSFHBWT and Fshb−/−
HSFHBdgc mice, their interspecies hybrid FSH heterodimer composition, number of N-linked glycans, FSH dimer assembly and secretion characteristics. Genetic rescue of Fshb−/− mice was achieved with HSFHBWT, but not HSFHBdgc transgene, confirming that N-linked glycans of hFSHβ subunit are essential for FSH heterodimer assembly and secretion in vivo. The positions of Asn-linked N-glycans on FSH α (Asn52 and Asn78) – and β -subunits (Asn7 and Asn24) are indicated.