Table 2.
Expression pattern of 28 effector proteins, including known Avr genes, during Leptosphaeria maculans compatible and incompatible interactions with canola.
| Gene name | Gene ID | Expression value (FPKM*) | Avr genes | Previously reported | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Topas-wild (compatible) | Topas-Rlm2 (incompatible) | ||||||||||
| 3 dpi | 5 dpi | 7 dpi | 11 dpi | 3 dpi | 5 dpi | 7 dpi | 11 dpi | ||||
| Gene_9004 | Lema_T038230 | 0.0 | 122.3 | 23963.3 | 2751.1 | 0.0 | 106.3 | 257.6 | 38.1 | 1 | |
| Gene_1613 | Lema_T076380 | 161.7 | 2525.2 | 11944.8 | 2194.9 | 79.6 | 1065.7 | 1667.9 | 435.4 | 1 | |
| Gene_1720 | Lema_T086760 | 0.0 | 299.4 | 8458.8 | 2802.2 | 7.9 | 744.5 | 1618.6 | 1428.4 | 1 | |
| Gene_11177 | Lema_T070880 | 77.5 | 928.2 | 6769.7 | 2786.3 | 10.6 | 106.3 | 487.2 | 375.2 | AvrLmJ1 | 1.2 |
| Gene_6961 | Lema_T119060 | 51.0 | 1646.3 | 6653.6 | 1346.6 | 32.0 | 899.8 | 627.7 | 263.3 | AvrLm11 | 2 |
| Gene_8487 | Lema_T033060 | 82.9 | 1626.7 | 5468.1 | 728.6 | 91.9 | 463.7 | 322.1 | 58.5 | 1 | |
| Gene_12203 | Lema_T064550 | 361.8 | 939.2 | 4087.3 | 3933.3 | 350.5 | 1046.2 | 470.3 | 385.3 | ||
| Gene_2817 | Lema_T081180 | 32.0 | 32.3 | 3930.2 | 1420.1 | 30.6 | 354.6 | 679.8 | 164.6 | 1 | |
| Gene_6375 | Lema_T113200 | 67.4 | 1117.1 | 3555.8 | 959.4 | 23.7 | 437.1 | 627.4 | 188.6 | 1 | |
| Gene_10780 | Lema_T049660 | 57.3 | 673.4 | 3553.0 | 875.5 | 29.0 | 108.0 | 205.8 | 134.1 | AvrLm1 | 2 |
| Gene_7223 | Lema_T121680 | 61.1 | 448.7 | 2982.2 | 1139.6 | 71.0 | 323.9 | 211.3 | 291.7 | 1 | |
| Gene_6114 | Lema_T020880 | 14.5 | 937.1 | 2479.8 | 722.8 | 14.4 | 145.6 | 8.9 | 71.3 | 1 | |
| Gene_2397 | Lema_T086290 | 23.6 | 237.1 | 2130.8 | 859.7 | 18.8 | 64.8 | 134.7 | 116.7 | AvrLm4-7 | 2 |
| Gene_7362 | Lema_T123070 | 0.0 | 87.8 | 1952.3 | 412.2 | 0.0 | 232.6 | 93.3 | 308.9 | 2 | |
| Gene_1698 | Lema_T086540 | 15.8 | 411.8 | 1775.8 | 607.6 | 18.6 | 230.1 | 110.7 | 118.2 | 1 | |
| Gene_170 | Lema_T001700 | 0.0 | 41.2 | 1695.1 | 1377.3 | 17.5 | 297.1 | 289.5 | 150.6 | ||
| Gene_6860 | Lema_T118050 | 385.1 | 636.1 | 1551.7 | 918.3 | 325.5 | 351.1 | 233.1 | 192.1 | 1 | |
| Gene_2728 | Lema_T080290 | 0.5 | 1.6 | 804.9 | 112.6 | 17.7 | 49.6 | 124.6 | 141.4 | ||
| Gene_5357 | Lema_T013310 | 225.6 | 210.0 | 574.7 | 236.9 | 137.6 | 65.9 | 43.7 | 21.3 | ||
| Gene_10809 | Lema_T049950 | 0.0 | 62.8 | 526.2 | 154.7 | 5.2 | 12.4 | 20.3 | 20.2 | AvrLm2 | 1 |
| Gene_8681 | Lema_T035000 | 0.0 | 296.1 | 466 | 56.8 | 0.0 | 75.9 | 83.1 | 157.4 | 1 | |
| Gene_7202 | Lema_T121470 | 19.3 | 116.8 | 453.4 | 211.5 | 7.3 | 45.3 | 22.4 | 49.2 | ||
| Gene_7203 | Lema_T121480 | 0.0 | 85.6 | 435.3 | 97.3 | 0.0 | 18.0 | 59.7 | 0.0 | 1.2 | |
| Gene_3097 | Lema_T095640 | 48.1 | 40.4 | 369.8 | 155.8 | 59.6 | 219.7 | 185.9 | 66.1 | 1 | |
| Gene_11572 | Lema_T058240 | 0.0 | 0.0 | 300.2 | 3.7 | 0.0 | 17.5 | 225.4 | 0.0 | ||
| Gene_9602 | Lema_T044210 | 0.0 | 56.1 | 136.2 | 54.6 | 0.0 | 19.9 | 3.3 | 1.1 | 1 | |
| Gene_11574 | Lema_T058260 | 0.0 | 0.0 | 130.6 | 2.9 | 0.0 | 0.0 | 110.4 | 16.6 | ||
| Gene_11156 | Lema_T070670 | 57.9 | 30.8 | 89.1 | 25.1 | 22.5 | 5.3 | 3.5 | 9.2 | ||
RNA-seq analysis was performed for L. maculans isolate D5 inoculated to Topas-wild (compatible) and Topas-Rlm2 (incompatible) canola genotypes.