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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Immunol Methods. 2016 May 6;436:1–15. doi: 10.1016/j.jim.2016.05.003

Fig. 7.

Fig. 7

Expression of transcripts encoding enzymes involved in the establishment and removal of modified DNA cytosine. A–C. qRT-PCR analysis of the relative transcript expression was performed on cDNA prepared from seven leukocyte types. Values are expressed as a scaled Relative Quantity (RQ) of transcript in each cell type using the dCT method. Letters designate significant differences of at least p < 0.05. A. Analysis of transcripts for DNMTs (Fig. 1). The RQ value for each cell type is presented as a scaled value of 104, 105, and 106 times their RQ value for DNMT1, DNMT3A, and DNMT3B, respectively. B. Analysis of transcripts for TETs (Fig. 1). The RQ value for each cell type is presented as a scaled value of 106, 105, and 105 times their RQ value for TET1, TET2, and TET3, respectively. C. Analysis of transcripts for GADD45s (Fig. 1). The RQ value for each cell type is presented as a scaled value of 105, 105, and 106 times their RQ value for GADD45A, GADD45B, and GADD45G, respectively.