Figure 2. Neurexin expression in mushroom body neurons is essential for nighttime sleep.

(A) Total nighttime sleep in flies with rescued neurexin expression using anatomically restricted GAL4 drivers. All rescue experiments were performed in the nrx^273/Δ83 background; flies carry one copy of the indicated drivers (n = 32). (B,C) Average sleep profiles for rescued and control flies, with curves plotted as 30 min moving averages (B); quantification of total nighttime sleep in rescued and control flies (C); n = 61. (D,E) Average sleep profiles for MB247-GAL4/UAS-nrx^RNAi and control flies, plotted as a 30 min moving average (D), and quantification of total nighttime sleep for each genotype (E); n = 32. (F) Cumulative sleep lost during 12 h of sleep deprivation and regained during subsequent recovery for 24 h in c739-GAL4/+;UAS-nrx^RNAi/+ flies and control flies (n = 16). (G) Model of the fly MB illustrating the different subsets of intrinsic Kenyon cells (KCs) within the lobes. Purple, αβ lobes; orange, α′β′ lobes; blue, γ lobes. (H) Total nighttime sleep in flies with depleted neurexin. Each GAL4 expression pattern is summarized at the top. A single copy of the driver was used for each GAL4 line (n = 32).