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. 2016 Dec 1;6:38246. doi: 10.1038/srep38246

Figure 7. The αβs neurons release both acetylcholine and short neuropeptide F to promote nighttime sleep, whereas α β c neurons release sNPF to maintain nighttime sleep.

Figure 7

(A) Expression of c739 in αβsp neurons is blocked by Cha-GAL80. The inset shows a horizontal cross-section through the vertical collateral at the level of the dashed line in each panel. Scale bar = 20 μm. (B,C) Averaged total sleep per night in c739-GAL4/+;UAS-nrxRNAi/+ and c739-GAL4i/+;Cha-GAL80/UAS-nrxRNAi flies, plotted as a 30 min moving average (B), and quantification of total nighttime sleep in each genotype (C) (n = 64). (D,E) Averaged total sleep per night in MB185B-GAL4/UAS-vAChTRNAi and control flies (D) (n = 64), and in MB594B-GAL4/UAS-vAChTRNAi and control flies (E) (n = 24), plotted as a 30 min moving average. (F) Quantification of total nighttime sleep in vAChT-RNAi flies and control flies. (G,H) Averaged total sleep per night in MB185B-GAL4/UAS-sNPFRNAi and control flies (G) (n = 32), and in MB594B-GAL4/UAS-sNPFRNAi and control flies (H) (n = 32), plotted as a 30 min moving average. (I) Quantification of total nighttime sleep in sNPFRNAi flies and control flies.