Figure 6. High EMR2-expressed neutrophils from cirrhotic patients represented an apoptotic phenotype and could not be rescued by opsonized E. coli incubation.

(A) Neutrophils from cirrhotic patients were more apoptotic than from healthy volunteers. (B) Neutrophils with high expression of EMR2 were also with high expression of annexin-V and high percentages of 7-AAD (C) Neutrophil from healthy volunteers or cirrhotic patients were incubated respectively with opsonized E. coli without/with anti-EMR2 antibody (2A1). E-coli could prolong the survival of neutrophils from healthy volunteers (decreased % of apoptosis) but not from cirrhotic patients. Ligation of EMR2 reversed the effect of neutrophil survival prolongation by E-coli from healthy volunteers but not from cirrhotic patients.