Figure 5.

Temperature dependence of specific activities calculated from photometric activity assays using 0.15 mg mL⁻¹ of purified CpuTA1 (A) or 0.1 mg mL⁻¹ of MgiTA1 (B) in 50 mM KPi, pH 8, at various T. The reaction mixtures (5 mM pyruvate, 5 mM (R)-1-aminotetralin, 0.1 mM PLP in buffer) were incubated for 5 minutes at the respective temperature before addition of pyruvate to start the reaction. The measurements were done in triplicate. Grey diamonds: CpuTA1, black triangles: MgiTA1.