Table 1. Summary of ANOVAs.

Quantitative trait	F (DFn, DFd)	P value
Expansion rate (A motility)	3.877 (8, 36)	0.0022
Expansion rate (S motility)	3.050 (8, 36)	0.01
Aggregate count	111.4 (8, 18)	<0.0001
Viable spore count	28.23 (8, 18)	<0.0001

A one-way analysis of variance (ANOVA) was performed for each quantitative trait. Degrees of freedom (DF) are calculated from the number of sublines (nine, numerator) and replicate experiments (three or five, denominator). Aggregate count data were normalized by log-transforming prior to analysis. Significant differences in subline means are indicated by P < 0.05.