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. 2016 Dec 1;6:38027. doi: 10.1038/srep38027

Figure 5. Secretion of p17 protein depends upon cleavage by cellular proteases.

Figure 5

(A) Evaluation of p17 secretion in the presence of inhibitors of different cellular proteases. Jurkat-Gag were cultured for 16 h in ELISA plates in the presence or absence of a cocktail containing inhibitors of different cellular proteases. Secreted p17 was quantified by cellular ELISA as in Fig. 1. Box plots represent statistical analyses of six independent experiments. Significance was assessed using Student’s t test (***P < 0.001). (B) The same experiments as in (A) were performed using inhibitors of specific cellular proteases as Pepstatin A, Bestatin, Aprotinin and Leupeptin or (C) different doses of the aspartyl protease inhibitor Pepstatin A. Box plots represent statistical analyses of six independent experiments. Significance was assessed using one-way ANOVA and the Bonferroni’s post-test was used to compare data; **P < 0.01, ***P < 0.001. (D) Confocal analysis of p17 localization in Pepstatin-A-treated cells (0.5 μM) shows that p17 accumulates at the plasma membrane. z-Stack sections and orthogonal z reconstitution are also shown. Scale bar, 10 μM.