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. 2016 Dec 1;6:38061. doi: 10.1038/srep38061

Figure 6. S. suis capsular polysaccharide interferes with cytokine production by CD4+ T cells in vitro.

Figure 6

Bone marrow-derived dendritic cells (bmDCs) were infected with either S. suis wild-type strain P1/7 or its non-encapsulated isogenic mutant ΔcpsF (MOI: 1) for 1 h. Extracellular bacteria were killed by antibiotic treatment and cultures washed prior to addition of freshly isolated splenic CD4+ T cells from naive mice (T cell: DC ratio of 5: 1). Cocultures were incubated for 48 h, resuspended in fresh medium containing 10 ng/ml of IL-2 for 72 h (resting period) and then transferred to anti-CD3 coated plates for 48 h. Supernatants were then collected and cytokines quantified by ELISA. Non-stimulated cocultures served as negative controls (C−) for basal expression. Data are expressed as mean ± SEM (in pg/ml) from 5 different experiments. #P < 0.05, indicates statistically significant differences compared to negative controls (C−). *P < 0.05, indicates statistically significant differences between cocultures infected with the wild-type strain P1/7 and those infected with the non-encapsulated mutant ΔcpsF.