Fig. 4. Cross-section confocal fluorescence image showing co-localization in live HeLa cells. (a) Ru-ER (70 μM) in red and ER Tracker Blue (1 μM) in blue shown and the corresponding plot demonstrating both compounds localize within the endoplasmic reticulum and are nuclear excluding, where (
) represents Ru-ER and (
) ER Tracker Blue. (b) Ru-NLS (40 μM) in red and DAPI nuclear stain (300 nM) in blue. The plot profile indicating both compounds co-localize within the nucleus, where (
) represents Ru-NLS and (
) DAPI. Ru-ER was excited using a 488 nm white light laser and the emission was collected between 590 and 700 nm. Ru-NLS was excited using a 470 nm white light laser and the emission was collected between 565 and 700 nm. DAPI was excited at 633 nm and the emission was collected between 637 and 730 nm.
