Fig. 7. Comparison of confocal versus STED images of (a) AlexaFluor 532, and (b) Ru-Arg8. The FWHM was obtained by fitting fluorescence-intensity to Gaussian distributions (OriginPro). Images of fixed HeLa cells dual stained with Ru-ER and Alexa Fluor 532. Line traces and the corresponding plot profile show the greatly improved resolving power of STED imaging (
) or (
) compared to confocal (
). Samples were fixed with 3.8% paraformaldehyde for 15 minutes, and then mounted with Prolong Gold for 24 h before imaging. Samples were excited using a 488 nm white light laser, and the 660 nm STED depletion. Data is raw with no post-processing performed.
