Fig. 8. Comparison CW-STED versus gCW-STED images of Alexa Fluor 532 (a) and Ru-ER (b) in a fixed HeLa cell. Line traces and the corresponding plot profile show the greatly improved resolving power of using gating (
) and (
) versus no gating (
). Samples were fixed with 3.8% paraformaldehyde for 15 minutes, and then mounted with Prolong Gold for 24 h before imaging. Samples were excited using a 488 nm white light laser, and the 660 nm STED depletion. Data is raw with no post-processing performed.
