Figure 6. Macrophage depletion promotes A→β reprogramming.

(A) Representative H&E staining of 3TF mice administered either saline or GdCl₃ and WT mice administered GdCl₃ at 7 days dox. Tubular complexes (inset) observed in pancreas of 3TF mice administered saline. (B) Representative Masson’s trichrome stain of 3TF mice administered either saline or GdCl₃ and WT mice administered GdCl₃ at 7 days dox. (C) Pancreatic immunofluorescence insulin staining of 3TF mice administered GdCl₃ at 7 days dox. mCherry⁺ cells that co-expressed insulin were observed. (D) Percentage of Insulin⁺ cells among mCherry⁺ cells at 7 days of dox. Three mice per time point and over 1,000 mCherry⁺ cells counted. Data are represented as mean ± SEM. (E) RT-qPCR analysis of Ins1 and Ins2 expression in 3TF mice given either saline or GdCl₃ after 7 days of dox. Fold change calculated against mRNA expression in uninduced acinar cells. Data are represented as mean ± SEM. **p < 0.001, student’s t-test. See also Figure S7 and Table S6.