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. 2016 Dec 1;13:201. doi: 10.1186/s12985-016-0655-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — PCR-Activated Virus Sorting (PAVS) workflow. Virus suspensions are encapsulated with PCR reagent and probe in double emulsion droplets, then thermal cycled and stained with SYBR Green. FACS recovers fluorescent droplets containing the viral species of interest, generating an enriched sample that is ready for downstream processing