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. 2016 Dec 1;13:201. doi: 10.1186/s12985-016-0655-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a T4, ФX174, and lambda virions are partitioned into droplets with TaqMan primers and probe specific for T4. After thermal cycling, the T4 sample has TaqMan signal while the ФX174 and lambda negative controls have no signal, demonstrating that digital droplet PCR specifically detects target viruses. b The fraction of TaqMan positive droplets in digital PCR for T4 is closely related to the input T4 concentration, showing that digital droplet PCR quantitatively measures viral concentration. Error bars indicate the standard deviation for triplicate measurements