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. 2016 Dec 1;13:201. doi: 10.1186/s12985-016-0655-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — a Location of TaqMan PCR Cy5 probe in the lambda genome is shown in red, FAM probes are shown in green, and the location of the ApaI restriction site is shown in black. b Representative image of multiplexed PCR emulsion on Lambda DNA. c Representative scatterplot of Cy5 and FAM intensities for Lambda DNA. d Fraction of multiplexed droplets for Lambda DNA undigested (blue), ApaI digested (red), and Fragmentase digested (green)