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. 2016 Dec 1;6:38139. doi: 10.1038/srep38139

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MEF cells were transfected with FLAG-STAT2, V5-IRF3 or Myc-FLAG-IRF7 plasmid as described before, and then infected with freshly grown PVM at the specified m.o.i. At 12 h and 24 h post-infection cells were analysed for the indicated proteins by immunoblot with a mixture of the corresponding antibodies. Note the reduction of STAT2 and IRF3, but not IRF7 (detected by FLAG antibody), by virus infection. Replication of the virus is confirmed by expression of the N protein in an inoculum- and time-dependent manner. U = uninfected cells.