Table 1.
Genotypes of 88 Vorderwald cattle at the rs876174537 polymorphism in COL7A1
| Animal group | N | Genotypes at the COL7A1 variant | ||
|---|---|---|---|---|
| CC | CT | TT | ||
| Calves with epidermolysis bullosa | 2 | - | - | 2 |
| Obligate carriers | ||||
| Dams of calves with EB | 7 | - | 7 | - |
| Sires of calves with EB | 7 | - | 7 | - |
| Healthy bulls used in artificial insemination | ||||
| Carriers of the EB-associated haplotype | 0 | - | - | - |
| Non-carriers | 21 | 21 | - | - |
| Haplotype state unknown | 51 | 50 | 1 | - |
Genotypes for the nonsense mutation (rs876174537) in COL7A1 were obtained in 88 animals of the Vorderwald cattle breed using Sanger sequencing including 14 parents of affected calves (obligate carriers). The seven sires of affected calves were also used in artificial insemination