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(A) mCherry and GFP fluorescent protein FRET pair spectral profiles. (B) FRET analysis of ~100% attachment of mCherry-Cbox/GFP-Wbox, ~50% attachment of mCherry-Cbox/GFP-Wbox, and 0% attachment of unmodified mCherry and GFP (C) Loading efficiency of mCherry-Cbox and GFP-Wbox simultaneously attached to clathrin triskelions. (D) Stability of GFP-Wbox and mCherry-Cbox simultaneously attached to clathrin triskelions in the presence of serum. (C&D) Data are represented as mean ± sd (n=3).
