Table 1.

Circulating half-lives, dose normalized AUC, and terminal half-lives of unmodified fluorescent proteins, CBP fluorescent proteins, and the fluorescent protein-clathrin conjugates. Statistical analysis was performed by means of an unpaired two-tailed t-test with *p < 0.05, **p < 0.01, and ***p < 0.001. In all statistical analyses the CBP attached to clathrin was compared to the corresponding CBPs.

Protein Construct	Half-Life ± SE (min)	Dose Normalized AUC ± SE (min/mL)	Terminal Half-Life ± SE (min)
Clathrin triskelions	53.5 ± 12.2	21.5 ± 1.4	597 ± 87.4
Unmodified mCherry	10.1 ± 1.8	4.9 ± 0.2	82.4 ± 10.2
Unmodified GFP	5.8 ± 2.3	4.5 ± 0.2	15.8 ± 1.0
GFP-Wbox	6.4 ± 0.4	4.6 ± 0.2	51.6 ± 5.0
mCherry-Cbox	4.3 ± 0.2	6.0 ± 0.6	102 ± 17.5
mCherry-Cbox Clathrin	17.1 ± 0.6***	11.4 ± 0.7***	1,130 ± 401**
GFP-Wbox Clathrin	28.1 ± 0.4***	11.4 ± 0.8***	733 ± 108 **