Fig. 1.

　We performed coronary angiography with or without the intracoronary administration of serotonin (Se) or acetylcholine (ACh) using a 6 F. handmade Judkins type catheter for measuring the left anterior descending coronary artery (LAD) diameter with simultaneously recordings of electrocardiograms (ECG), aortic (Ao) blood pressure, and the change of LAD blood flow in anesthetized pigs. Next, balloon denudation was performed in the LAD just distal to the bifurcation of the LAD and the left circumflex coronary artery using a 2F Fogarty catheter. These procedures were performed every 2 weeks (W). We also observed hemodynamics and cardiac ischemic events under the conscious state with a telemetry system in the pigs.