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. 2016 Dec 1;7:299. doi: 10.3389/fphar.2016.00299

FIGURE 5.

FIGURE 5

Summary of the in vitro effects of Gly300Ser cysteinyl leukotriene 1 (CYSLTR1) receptor and Met201Val on CYSLTR2 receptor signaling compared with wild type (WT). (A) Cysteinyl leukotriene D (LTD) concentration-response curve for CysLT receptors in transfected cells. Inositol triphosphate (InsP) generation assay of the variants and WT forms of the CYSLTR1 receptor. both 300Ser and 206Ser variants’ EC50 were significantly different from WT. The concentrations of LTD required to produce the InsP effect were much higher than those used in the [Ca2+] assay shown in (B), in which calcium flux was assayed for the variants and WT and variant forms of the CYSLTR1 receptor challenged with LTD. The resulting changes in intracellular calcium concentrations were measured as fluorescence maximum. For LTD4, the Met201Val variant (○) had a significantly greater EC50 compared to WT (■), while the Ser236Leu(Δ) and Ala293Gly/Arg316Lys (®) variants were not different. However, the Ala293Gly/Arg316Lys variant showed decreased efficacy (V; adapted from Thompson et al., 2014a).