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. 2016 Dec 1;7:299. doi: 10.3389/fphar.2016.00299

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Copyright © 2016 Thompson, Capra, Clunes, Rovati, Stankova, Maj and Duffy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding of LTD4 to the CYSLTR1-WT receptor and its variants in transiently transfected HEK293 cells. Forty-eight hours post-transfection, 3H-LTD4 was added to the cells for 1 h, in the presence or absence of non-radioactive LTD4. Following several washes, the radioactivity was measured in a beta-counter (n = 3). Specific binding was calculated by subtracting the non-specific binding (in presence of competition from radioactive LTD4) from the total binding measured (Yaddaden et al., 2016).