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Inositol phosphate production in CYSLTR1-transfected cells. Forty-eight hours post-transfection, cells were stimulated for 30 min with LTD4 or LTC4 at 0.01–100 nM. IP-1 production was measured using an antibody against IP-1 and a fluorescent FRET IP-1 conjugate competitor. (A) IP-1 production by CYSLTR1-transfected HEK-293 cells in response to LTD4 (B) and to LTC4. Two-way ANOVA analysis; n = 6; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (adapted from Yaddaden et al., 2016).
