FIGURE 9.

Confocal microscopy imaging of cysteinyl-leukotriene 1 (CYSLTR1) WT and variant receptors in transiently transfected cells. Cells were fixed and immunofluorescently stained with anti-CYSLTR1R antibody (green) followed by Alexa 488 goat anti-rabbit antibody (red) as described under Methods section. Superimposed images of immunoreactivity of (a) WT CYSLTR1 receptor, (b) Gly300Ser CYSLTR1 receptor variant or (c) Ile206Ser CYSLTR1 receptor variant, in basal condition. One representative experiment is shown (adapted from Thompson et al., 2007).