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. 2016 Dec;196(3):437–447. doi: 10.1016/j.jsb.2016.09.011

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — USP12/UAF1 has 1:2 stoichiometry. A) SEC-MALLS analysis of USP12_FL-Ub/UAF1₅₈₀ (8 μM) shows a molecular weight corresponding to a 1:2 complex. B) SEC-MALLS analysis of USP12_FL-Ub/UAF1_FL at high concentration (42 μM) shows a dynamic equilibrium between high molecular weight species and the 1:2 complex in agreement with the SAXS data. C–E) Experimental SEC-SAXS curve of USP12_FL-Ub/UAF1_FL fits better with the scattering curve based on the structure of the 1:2 complex as compared to the 1:1 complex at either of the two interfaces. F) OLIGOMER fit of the Experimental SEC-SAXS curve of USP12_FL-Ub/UAF1_FL.