Table 1.
Crystallography details.
| Data collection | |||
|---|---|---|---|
| USP46-UbVME | UAF1580 | USP12-UbPRG/UAF1580 | |
| Wavelength (Å) | 0.93 | 0.92 | 1.00 |
| Resolution (Å) | 28.41–1.85 (1.90–1.85) | 48.97–2.30 (2.35–2.30) | 47.41–2.79 (2.92–2.79) |
| Space Group | C 2 2 21 | P 2 21 21 | I 2 2 2 |
| Unit Cell a, b, c (Å) | 91.95 104.66 135.31 | 73.30 131.60 148.67 | 103.68 152.82 182.93 |
| CC1/2 | 0.999 (0.720)∗ | 0.999 (0.585) | 0.995 (0.638) |
| Rmerge | 0.12 (0.84) | 0.05 (1.0) | 0.08 (0.72) |
| Rpim | 0.04 (0.84) | 0.06 (0.57) | |
| I/σI | 17.68 (2.0) | 16.1 (1.4) | 10.4 (1.6) |
| Completeness (%) | 98.8 (99.7) | 99.6 (95.9) | 99.3 (94.8) |
| Redundancy | 3.56 (3.3) | 4.5 (4.1) | 4.5 (4.3) |
| Refinement | |||
| Unique Reflections (nr) | 55,291 | 64,580 | 36,148 |
| Atoms total (nr) | 3442 | 8586 | 7290 |
| Protein atoms (nr) | 3192 | 8195 | 7266 |
| Solvent atoms (nr) | 250 | 391 | 24 |
| B-factors | 31.9 | 58.3 | 72.4 |
| TLS groups | 4 | 4 | 4 |
| Rwork/Rfree (%) | 16.6/19.4 | 18.5/22.4 | 20.8/25.9 |
| Rmsd bond lengths (Å) | 0.017 | 0.007 | 0.014 |
| Rmsd bond angles (°) | 1.758 | 1.235 | 1.642 |
| Ramachandran (%) Preferred/outliers | 97.89/0 | 97.27/0.30 | 95.5/0.34 |
| Molprobity score | 1.01 | 0.82 | 1.77 |
| PDB code | 5L8H | 5L8E | 5L8W |
High resolution shell in parentheses; (∗ – Reprocessed with aimless).