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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Leukemia. 2016 Feb 5;30(5):1155–1165. doi: 10.1038/leu.2016.2

Figure 7.

Figure 7

BCOR loss of function mutations are associated with enhanced myeloid cell differentiation and dysfunction of PRC1 in MDS and AML cells of patients. (a–c) BM cells were collected from 6 male AML patients with WT BCOR (WT) and 6 male AML patients with BCOR nonsense mutation (Mut). Gene expression analysis (Affymatrix microarray) was performed, and GSEA comparison were made of Mut and WT cells for enrichment (left panels) or depletion (right panels) of up-regulated and down-regulated genes associated with myeloid cell differentiation (a), HSC (b), and PRC1 targets (c). The NES and P values are indicated in each plot. (d) qRT-PCR results for HOXA9 expression from a total 73 MDS patients with normal karyotyping. BM cells were collected from 12 males with mutant BCOR (11 of which had destructive mutations), 26 males with wild type BCOR, 11 females with mutant BCOR (7 of which had destructive mutations) and 24 females with wild type BCOR. Extracted RNA samples were subjected to qRT-PCR analysis. Each dot represents one sample and data represent mean ± SD with P values (two-tailed, Unpaired t test) indicated.