Figure 6. Effects of dexmedetomidine (DEX) treatments on caspase 3, caspase 9 activities, mitochondrial membrane depolarization (JC-1) and intracellular ROS production through TRPV1 and TRPM2 channel activities in HIPPO neurons of ischemia (ISC)-induced rats [mean ± SD and n = 12 except control (n = 8)].

HIPPO neurons of control, sham, DEX, DEX+ACA, ISC, ISC+ACA, ISC+DEX and ISC+DEX+ACA groups were in vitro stimulated in TRPM2 (6A and C) and TRPV1 (6B and D) experiments by CHPx (1 mM) and CAP (0.01 mM) although they were inhibited by ACA (0.025 mM) and CPZ (0.1 mM) respectively. (^ap ≤ 0.05 and ^bp ≤ 0.001 vs control, sham and DEX groups. ^cp ≤ 0.001 vs DEX+ACA and DEX+CPZ groups. ^dp ≤ 0.001 vs ISC group. ^ep ≤ 0.05 vs ISC+DEX group).