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. 2016 Oct 18;28(1):31–39. doi: 10.1097/CAD.0000000000000421

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Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.

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Fig. 1 — Cloning, expression, and purification of GST and RGD-IFN-α2a-core fusion proteins. (a) Schematic diagram of pGEX-4T-RGD-IFN-α2a(300)-core (Q1), pGEX-4T-RGD-IFN-α2a-core (Q2), and pGEX-4T-IFN-α2a (NR). (b) Purified Q1 protein. M: protein marker; 1: pGEX-4T-RGD-IFN-α2a(300)-core without being induced; 2: pGEX-4T-RGD-IFN-α2a(300)-core induced by IPTG; 3: purified Q1 protein. (c) Purified Q2 and NR proteins. M: protein marker; 1: pGEX-4T-RGD-IFN-α2a-core without being induced; 2: pGEX-4T-RGD-IFN-α2a-core induced by IPTG; 3: purified Q2 protein; 4: purified NR protein. GST, glutathionine S-transferase; IFN, interferon; NR, nitrate reductase; RGD, arginine–glycine–aspartic acid.