Fig. 3.

Significant contribution of T_CM with Th17 and Th1Th17 phenotypes to the pool of HIV DNA reservoir.

ART, antiretroviral therapy; T_CM, central memory T cell. Peripheral blood mononuclear cell from HIV⁺ on ART and HIV individuals were stained with a cocktail of CD3, CD4, CD45RA, CCR6, CCR4, CCR7, and CXCR3 Abs. (a) Shown is the gating strategy for the identification of T_CM cells with Th17 (CCR6⁺CCR4⁺CXCR3⁻), Th1Th17 (CCR6⁺CCR4⁻CXCR3⁺), Th2 (CCR6⁻CCR4⁺CXCR3⁻), and Th1 (CCR6⁻CCR4⁻CXCR3⁺) phenotypes. The frequency of the four T_CM subsets was compared between HIV⁺ on ART and HIV⁻ controls (b). Highly pure T_CM subsets were sorted by flow cytometry from HIV⁺ on ART individuals (Suppl. Figure 3) and integrated HIV DNA levels were quantified. Shown are levels of integrated HIV DNA in subsets from n = 5 individual donors (c) and statistical analysis in all donors (d). The relative contribution of the four T_CM subsets to the pool of integrated HIV DNA within T_CM cells was calculated considering the frequency of each subset as described in Fig. 2f legend. (e). Indicated in the graphs are Mann-Whitney (b) and Friedman and Dunn's multiple comparison test P values (^∗, P < 0.05; ^∗∗, P < 0.01; ^∗∗∗, P < 0.001) (d and e).