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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cell Rep. 2016 Nov 15;17(8):2015–2027. doi: 10.1016/j.celrep.2016.10.070

Figure 5. Adipocyte Snail1 mediates downregulation of adipose ATGL in obesity.

Figure 5

A-B. C57BL male mice (7 weeks) were fed a standard chow diet or a HFD for 13-14 weeks; ob/ob male mice were fed a normal chow diet. A. Snail1 and ATGL expression (normalized to 36B4 expression) was measured in eWAT by qPCR. Chow: n=5, HFD: n=6, ob/ob: n=4. B. eWAT extracts were immunoblotted with the indicated antibodies. C. AKO and Snail1flox/flox mice were fed a HFD for 8 weeks (females) or 30 weeks (males). Gonadal WAT extracts were immunoblotted with the indicated antibodies. D-E. AKO and Snail1flox/flox mice were fed a HFD for 8 weeks (females) or 20 weeks (males). Gonadal WAT explants were stimulated with isoproterenol (1 μM), and FFAs and glycerol release was measured (normalized to protein levels). AKO males: n=6, Snail1flox/flox males: n=6, AKO females: n=3, Snail1flox/flox females: n=5. F. AKO and Snail1flox/flox male mice (7 weeks) were fed a HFD for 20 weeks, and injected with CL316243 (1 mg/kg body weight). Plasma FFA and glycerol levels were measured. n=11. The values are mean ± sem. *p<0.05.