Fig. 3. DENV-infected cells display increased mitochondrial respiration.
A. OCR in uninfected and DENV-infected Huh7 cells at 48h post-infection (MOI of 1) were measured in real time under basal conditions and in response to sequential injections of oligomycin (1 μM), FCCP (1 μM) and antimycin A/rotenone (0.5 μM) using XF24 Seahorse analyzer. Data are shown as percent of baseline OCR relative to first measurement from uninfected cells and represent mean +/− SEM of three independent experiments with at least five biological replicates per condition and measurement. B. Mitochondrial function parameters were analyzed according to Seahorse Bioscience instructions and represented as fold change in OCR relative to uninfected cells. Data are shown as mean +/− SD, analyzed with unpaired Student-t test.