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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Virology. 2016 Nov 4;500:149–160. doi: 10.1016/j.virol.2016.10.022

Fig. 6. Mitochondrial fission inhibits DENV replication.

Fig. 6

A. Huh7.5 cells were transfected with dsiRNA against the untranslated region of Drp1. Cells were transfected with plasmids expressing Drp1 wildtype, phosphomimetic mutants of human Drp1 (S616D and S637D), phosphodeficient mutants (S616A and S637A) and dominant negative protein Drp1 K38A and infected with DENV (MOI of 0.1) for 24h. Western blots were probed with antibodies directed against NS3 and NS4b proteins and equal protein loading was assessed using an antibody against β-actin. Blot is representative of at least three independent experiments. B. For quantification of protein levels, blots of three independent experiments were evaluated after normalization to β-actin and expressed as mean +/− SEM. Statistical analysis was performed using unpaired Student-t test. C. DENV titers from collected supernatants of Huh7.5 cells expressing indicated Drp1 proteins were analyzed using an ELISpot assay. Titers are representative of independent experiments (n=3).