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Coverslip versus CLMA. Immunocharacterization of human induced pluripotent stem neuralized AF22 cell line by CLMA technology. AF22 cells grown in undifferentiated and differentiated conditions were expanded in flasks and collected by trypsinization for CLMA immunocharacterization or grown on coverslips. Undifferentiated and differentiated AF22 cells were analyzed for neural precursor markers Nestin and Sox2, and for the differentiation markers βIII-tubulin and GFAP.
