Figure 2.
Cell harvesting by mechanical scraping. No further subdivisions (lines A–X) were indicated in the figure. Human fetal neural stem cells CB660SP were differentiated toward neural lineage, and cell pellets were collected at days 0, 7, 14, and 21 of differentiation using mechanical scraping approach to better preserve cell morphology. Immunofluorescence analysis of pluripotency markers (Sox2, Nestin, and SEL1L) and neuronal differentiation markers (GFAP and βIII-tubulin) is shown in addition to Vimentin, a cytoskeleton marker used as control of intermediate filament. Scale bars: A–X = 200 µm. Abbreviation: CLMA, cell line macroarray; GFAP, glial fibrillary acidic protein.