Table 1.
Primary Antibodies Used in the Immunofluorescence Protocol.
| Antibody | Antigen Cellular Localization | Monoclonal Antibody Source and Ig Type | Dilution or Final Concentration | Supplier and Catalog Number |
|---|---|---|---|---|
| α-Sox2 | Nuclear | Rabbit IgG | 1:300 | Merck Millipore (Milan, Italy); AB5603 |
| α-Nestin | Cytosolic | Rabbit IgG | 1:200 | Merck Millipore; AB5922 |
| α-Oct3/4 | Nuclear | Mouse IgG | 1:50 | Santa Cruz Biotechnology (Heidelberg, Germany); SC5279 |
| α-Nanog | Nuclear | Rabbit IgG | 1:50 | Abcam; AB21624 |
| α-SEL1L | Ubiquitary | Mouse IgG | 5 µg/ml | Orlandi et al.15 |
| α-βIII-Tubulin | Cytosolic | Rabbit IgG | 1:100 | Sigma Aldrich; T2200 |
| α-GFAP | Cytosolic | Rabbit IgG | 1:1000 | Sigma Aldrich; G9269 |
| α-Vimentin | Cytosolic | Mouse IgG | 30 µg/ml | Sigma Aldrich; V6389 |
Abbreviation: GFAP, glial fibrillary acidic protein.