Figure 2.

snRNA-binding properties of G5N. (A, left panel) Schematic diagram showing U4 snRNA fragments used for electrophoretic mobility shift assay (EMSA) experiments. The Sm sequence is highlighted with red-shaded boxes. The 13-nt RNA fragment used for subsequent studies is numbered following a scheme indicated below the nucleotide sequence. (Right panel) EMSA results of the binding of G5N to the indicated RNA molecules. A 3:1 protein to RNA molar ratio was used. (B) The binding of G5N to the 13-nt RNA fragment and its base substitution derivatives. Each nucleotide of the Sm site and a 5′ adenine were individually changed to a cytosine, and protein to RNA molar ratios of 1, 2, and 3 were used in EMSA.