Figure 5.

PDGFRα and PDGFRβ physically interact in the craniofacial mesenchyme. (A–D′) Interaction of PDGFRα and PDGFRβ in E13.5 craniofacial tissue as assessed by fluorescence analysis following application of the PLA. PDGFRα/β interactions were significantly increased in the mesenchyme (A,A′) as compared with the epithelia of experimental sections as well as compared with a technical negative control without the addition of primary antibodies (B,B′) and conditional knockout tissues in which Pdgfra (C,C′) or Pdgfrb (D,D′) had been ablated in the NCC lineage. Puncta are indicated in inverse grayscale (A,B,C,D) and by Cy3 fluorescence (red; A′,B′,C′,D′). Nuclei were stained with DAPI (blue; A′,B′,C′,D′). Bars, 100 µm. (E) Scatter dot plot depicting the number of puncta per 50-µm² area detected in the mesenchyme (red) or epithelia (blue) for each condition. Data are presented as mean ± SEM. (***) P = 0.001; (****) P < 0.0001.