Figure 5. Fiber-deprived gut microbiota contributes to lethal colitis by Citrobacter rodentium.
(A) Fecal C. rodentium levels over time. Data are shown as average ± SEM. Student’s t-test; FR (SM+Cr) group vs. FF (SM+Cr) group (bottom statistics labels) and FR (Cr) vs. FF (Cr) (top statistics labels). Data in Figure 5A–5G are from Experiment 2B.
(B) Relative abundance of C. rodentium in fecal samples over time. Data are shown as median ± IQR. Wilcoxon test.
(C) Weight changes in the four groups of mice. Values are shown as average ± SEM. One-way ANOVA, FF diet group (with SM) vs. other groups.
(D) Survival curves for the four groups of mice. One-way ANOVA with Tukey’s test.
(E) Representative images of unflushed ceca after Hematoxylin and Eosin (H/E) staining highlighting major differences in hyperplasia (indicated with arrows in the FR group, where hyperplasia is patchy and infrequent). Scale bars, 5 mm.
(F) Images of representative H/E stained colonic thin sections depicting differences in hyperplasia between two groups. Scale bars: low power, 500 μm; high power, 50 μm.
(G) Measurements of inflamed tissue area in different intestinal segments. n = 5 mice/group except that n = 4 mice were used for FF (SM+Cr) group. Values are shown as average ± SEM. Statistically significant differences are shown with letters within each intestinal segment; p < 0.0002; One-way ANOVA with Tukey’s test.