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. Author manuscript; available in PMC: 2017 May 28.
Published in final edited form as: Nat Plants. 2016 Nov 28;2:16179. doi: 10.1038/nplants.2016.179

Figure 3. Bimolecular fluorescence complementation and Yeast 2-Hybrid assays demonstrating PpSMF1 and PpSCRM1 protein-protein interactions.

Figure 3

(a) Representative bright-field, fluorescence and overlay/merged images of BiFC analysis showing pairwise combinations of bHLH constructs, each fused with a complementary, half-YFP molecule (nYFPn fusion and YFPc fusions, respectively). In the intact Allium cepa epidermis using bimolecular fluorescent complementation (BiFC), PpSMF1 and PpSCRM1 showed strong heterodimerization in the nuclei. Controls are described in Supp. Info. Fig 9. Scale = 100 μm. (b–d) Yeast two-hybrid analysis: (b) Growth on minimal medium. (c) Growth on stringent selection medium. Blue indicates reporter activation. (d) Key to patch plate assays is shown in (b) and (c).