Table 1. XIP binding on ComR WT and ComR mutant proteins as measured by ITC.
ComR | stoichiometry | KD (μM) |
---|---|---|
WT | 0.8 | 0.010 ± 0.001 |
K100A | 0.8 | 1.175 ± 0.245 |
T90A | 0.7 | 0.244 ± 0.108 |
Y91A | 0.9 | 0.141 ± 0.033 |
R92A | 1.2 | 0.277 ± 0.023 |
F171A/Y174A | 1.1 | 0.087 ± 0.009 |
K87A/K246A | 0.8 | 0.009 ± 0.003 |