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. 2016 Dec 1;12(12):e1005955. doi: 10.1371/journal.ppat.1005955

Fig 6. C6 can inhibit induction of ISRE-luciferase by a constitutively active ISGF3 mimic, IRF9-S2C.

Fig 6

HEK293T cells were transfected with plasmids encoding firefly luciferase under the control of an ISRE promoter, a constitutively expressed renilla luciferase and the other proteins shown. Cells were harvested after 24 h and the expression of firefly luciferase was measured and normalised to renilla luciferase expression. Results show the fold induction of firefly luciferase relative to the EV only control. Immunoblot analysis shows expression of IRF-9-S2C and the V5-tagged proteins in each sample. Immunoblots were performed at least twice and a representative figure is shown. Positions of molecular mass markers are shown to the left of the immunoblots. ****p<0.0001.