Skip to main content
. 2016 Dec 1;12(12):e1005955. doi: 10.1371/journal.ppat.1005955

Fig 7. C6 does not prevent the binding of IRF9-S2C or endogenous STAT2 to the ISRE.

Fig 7

HEK293T cells were transfected with plasmids expressing IRF9-S2C (lanes 1–9) and either V5-tagged C6 (lanes 1, 4 and 7), NiV-V (lanes 2, 5 and 8) or GFP (lanes 3, 6 and 9). Sixteen hours post transfection cells were harvested and lysates were incubated with polyd(I:C) for 30 min. Biotin-labelled ISRE probes (ISREcore lanes 4–6, and ISRErandom lanes 7–9) were then added for 1.5 h before addition of streptavidin agarose beads for a further 3.5 h. Beads were washed four times and proteins were eluted and subjected to SDS-PAGE and immunoblotting (A). Band intensities were quantified and normalised to Ku70 immunoprecipitation (B). Immunoblots were performed at least twice and a representative figure is shown. Positions of molecular mass markers are shown to the left of the immunoblots. **p<0.01.