Fig 2. In vivo myelomonocytic cell dynamics and their contribution to ECM-mediated pathology.

(A) Representative maximal projections of 3D time lapses were obtained from the thinned skull window (top) and corresponding ear (bottom) of naïve, d5 p.i., and d6 p.i. LysM^gfp/+ mice. Note the accumulation of myelomonocytic cells (green) along brain and ear vessels (red) visualized with quantum dots (n = 3–4 mice per group). See corresponding S1 Movie. (B) A representative 3D time lapse of the brain was obtained from a LysM^gfp/+ mouse infected with PbA-OVA-mCherry at d6 p.i. Myelomonocytic cells (green) were observed phagocytosing parasites (red) attached to the lumen of cerebral blood vessels (blue) (denoted with white arrows). Time points in the image series are denoted in minutes. See corresponding S3 Movie. (C) Survival curve for PbA-infected wild type mice (black line) and CCR2^-/- mice that were depleted of neutrophils following i.p. injection of anti-Ly6G at day -1 (red line) (n = 4–5 mice per group). (D) Representative brains from wild type (top) and CCR2^-/- (bottom) mice depleted of neutrophils at d-1. Mice were injected i.v. with Evans Blue dye at d6 p.i. (E) Fluorometric quantification of data shown in (D). Data are represented as mean ± SD (n = 5 mice per group). All data in this figure are representative of two independent experiments. Asterisks denote statistical significance (*P < 0.05).