Fig 1. Generation of TSPO KO mice.

(A) Schematic of generation of TSPO Floxed mice and conditional KO mice by Cre-LoxP System. Conditional TSPO KO mice were generated by targeting exon 2 and exon 3 of the mouse genomic locus, a NEO selection cassette Floxed by Frt sites. (B) Schematic of crossing program for TSPO global knockout mice, Protamine-Cre is a transgenic mouse where Cre recombinase expression is driven by sperm specific protamine promotor. (C) Genotyping of TSPO Floxed mice and KO mice by a TSPO genotyping primer pair 1 & 3, and Flp primers. (D) Genotyping of TSPO Floxed mice and KO mice by three TSPO genotyping primers 1, 2 and 3, and Protamine-Cre primers.